Structural and biochemical characterization of Acinetobacter baumannii ZnuA

Saleh Alquethamy; Katherine Ganio; Zhenyao Luo; Sheikh I Hossain; Andrew J Hayes; Thomas Ve; Mark R Davies; Evelyne Deplazes; Bostjan Kobe; Christopher A McDevitt

Journal article

Structural and biochemical characterization of Acinetobacter baumannii ZnuA

Saleh Alquethamy, Katherine Ganio, Zhenyao Luo, Sheikh I Hossain, Andrew J Hayes, Thomas Ve, Mark R Davies, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

Journal of Inorganic Biochemistry | Elsevier | Published : 2022

DOI: 10.1016/j.jinorgbio.2022.111787

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Abstract

Acinetobacter baumannii is a Gram-negative nosocomial pathogen associated with significant disease. Crucial to the survival and pathogenesis of A. baumannii is the ability to acquire essential micronutrients such as Zn(II). Recruitment of Zn(II) by A. baumannii is mediated, at least in part, by the periplasmic solute-binding protein ZnuA and the ATP-binding cassette transporter ZnuBC. Here, we combined genomic, biochemical, and structural approaches to characterize A. baumannii AB5075_UW ZnuA. Bioinformatic analyses using a diverse collection of A. baumannii genomes determined that ZnuA is highly conserved, with the binding site comprised by three strictly conserved histidine residues. The s..

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University of Melbourne Researchers

Andrew Hayes Author

Mark Davies Author

Christopher McDevitt Author

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Awarded by Australian Research Council (ARC) LIEF Grant

Awarded by National Health and Medical Research Council (NHMRC)

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Funding Acknowledgements

This research was undertaken with the assistance of resources and services from the National Computational Infrastructure (NCI), which is supported by the Australian Government and by resources provided by The Pawsey Supercomputing Centre with funding from the Australian Government and the Government of Western Australia. This research was also undertaken using the LIEF HPC-GPGPU Facility hosted at the University of Melbourne. This Facility was established with the assistance of Australian Research Council (ARC) LIEF Grant LE170100200. This work was supported by the National Health and Medical Research Council (NHMRC) grants 1071659 to B.K., 1122582 to C.A.M., and 1180826 to B.K. and C.A.M. E.D. is a UTS Chancellor's Postdoctoral Fellow, B.K. is an ARC Laureate Fellow (FL180100109), and C.A.M. is an ARC Future Fellow (FT170100006). The content of this study is solely the responsibility of the authors and does not necessarily represent the official views of the funding bodies.